in vitro compartmentalization

Mix up genomic library with cell-free expression system and oil.
End up with oil droplets with DNA plus expression system.

Advantages? Not sure

Apparently better than other in vitro selection techniques such as phage display/ribosome display/mRNA peptide fusion/SELEX (would like to read about these too)

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Paper on DNA shuffling of Fe-Fe hydrogenases
-> heterologous expression of h2ase in E coli:D
Looks to be similarly possible for Ni-Fe.

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Directed evolution of oxygenases:
Table 1 compares selection methods

FACS pretty good (10^7). Phage display probs wouldn't work cos the protein wouldn't fold properly. Solid media a bit iffy, but I think it would work well if there was a selection pressure on the cells.

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